Vitamin D plays a role in regulating the body's levels of calcium and phosphate
Uncategorized Jan 24
By menhc 0 Comments

Test for 25-hydroxy vitamin D

Calcidiol; 25-hydroxycholecalciferol test; 25-OH vitamin D test
Measuring the amount of vitamin D in your body is most accurately done using the 25-hydroxy vitamin D test.


Vitamin D plays a role in regulating the body’s levels of calcium and phosphate.

How the Exam is Conducted?

You must provide a sample of blood.

How to Get Ready for the Exam
You don’t usually have to fast. Nevertheless, the laboratory and the testing procedures employed will determine this. Observe any guidelines on not eating in advance of the exam.


The Test’s Experience

Some people have mild to moderate pain when the needle is inserted to take their blood. For others, it only hurts or pricks. There can be some throbbing afterwards.


The Reason for Conducting the Test

The purpose of this test is to measure the amount of vitamin D in your blood. It is typically not advised to screen for low vitamin D levels in any adult, including those who are pregnant.

On the other hand, people who are more susceptible to vitamin D insufficiency, such as those who:

Are over 65 years old? (As we age, we produce less vitamin D through our skin, and our gut absorbs less of it.)
Are obese (or have undergone bariatric surgery to lose weight)?
are taking specific medications, such as phenytoin.
possess thin bones or osteoporosis.
Get little exposure to the sun.
suffer from conditions like ulcerative colitis, Crohn’s disease, or celiac disease that affect their ability to absorb vitamins and nutrients from their intestines.

Typical Outcomes

The unit of measurement for the normal range of 25-hydroxy vitamin D is nanograms per milliliter, or ng/mL. Numerous professionals advise a concentration of 20–40 ng/mL. Some advise a concentration of 30 to 50 ng/mL.


Common measures for test results are shown in the above instances. The normal value ranges in various laboratories may differ slightly. Certain labs test different samples or employ different metrics. Discuss the significance of your individual test results with your physician, as well as whether vitamin D supplements might be necessary.


The way these tests are reported has left a lot of people perplexed.
The vitamin D that your body produces on its own, that you absorb from an animal source (such as fatty fish or liver), or that you take as a supplement is known as 25-hydroxy vitamin D3, or cholecalciferol.

The form of vitamin D you have absorbed comes from supplements or foods enriched with plant-based vitamin D; it is also known as 25-hydroxy vitamin D2 (ergocalciferol).

In the body, cholecalciferol and ergocalciferol function similarly. The total amount of 25-hydroxy vitamin D in your blood is the crucial value.

The Significance of Abnormal Results

A vitamin D insufficiency may be the cause of a lower-than-normal level and can arise from:

  • Darkly pigmented skin, avoiding the sun, or regularly wearing high-SPF sunscreen
  • Inadequate intake of vitamin D in the diet
  • Ilnesses of the kidneys and liver
  • Inadequate absorption of food
  • Usage of specific medications, such as rifampin, phenytoin, and phenobarbital

Poor absorption of vitamin D as a result of advanced age, weight-loss surgery, or disorders affecting the body’s ability to absorb fat.


African American children are more likely to have low vitamin D levels, particularly in the winter, as do exclusively breastfed infants.
Hypervitaminosis D, a disorder caused by an overabundance of vitamin D, could be the cause of a higher-than-normal level. The most frequent cause of this is overdoing excessively on vitamin D. Hypercalcemia, or an excess of calcium in the blood, may develop from it. Kidney damage and a host of symptoms result from this.


The danger associated with having your blood drawn is minimal. The size of arteries and veins varies from person to person and from side to side of the body. It could be harder to draw blood from certain people than from others.
There are a few more minor hazards that come with having blood drawn, such as:

⦁ Excessive bleeding

⦁ Multiple punctures to find veins

⦁ Lightheadedness or fainting

⦁ Hematoma (blood pooling under the skin)

⦁ Infection (a minimal risk any time the skin is broken).


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